The 2025 Segunda Federación play-offs ("Playoffs de Ascenso" or "Promoción de Ascenso") are the final play-offs for promotion from 2024–25 Segunda Federación to the 2025–26 Primera Federación. 
Format.
Twenty teams will participe in the promotion play-off. Each of the five groups of the Segunda División RFEF are represented by the four teams that finished the regular season between the second and fifth positions. In the draw for the first stage, the participating teams were assigned to pots corresponding to their final regular season position. While avoiding matches between teams from the same regular season group, second-place finishers were drawn against fifth-place finishers while teams that finished third would play teams that finished fourth. The same draw process was repeated, to the extent that it would be possible, in the draw for the second round.
The five winning clubs of the second stage will reach promotion to Primera RFEF and will accompany the five group champions who had already achieved their promotion.
The final two relegation spots (of 27 total) will also be determined via play-offs. The four 13th-place finishers with the lowest point totals will be drawn into two single-leg matches, with the winners securing survival in the Segunda División RFEF and the losers being relegated to the Tercera División RFEF. The two match-ups will be selected through a random draw and hosted at a venue chosen from among the stadiums selected to host the promotion play-offs.
Relegated teams.
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