The 2024–25 European Aquatics Challenger Cup was the 3rd edition of a water polo competition organised by European Aquatics. It acts as the third tier, below the LEN Champions League and the LEN Euro Cup. The reigning champions are Galatasaray, but they won't defend their title as they didn't enter. 
This is the last season where the Challenger Cup is the third tier tournament in European water polo, with the Conference Cup taking its place.
Dutch side, GZC Donk, won their first title after defeating Lithuanian team, EVK Zaibas, in the final.
Format.
16 teams are grouped into 4 pools of four clubs. The top 2 from every pool advances to the quarter-finals, followed by semi-finals and finals.
Rankings.
Only teams below the top 12 can enter this competition.
Teams.
No countries ranked in the top 12 of the LEN men's club rankings can take part in this tournament.
Qualification round.
In the qualification round, teams play in a round robin format. The top 2 in each group advance. Due to the lack of clubs wanting the host the groups, Nováky and Sliema hosted two groups.
Draw.
The draw was on 16 September 2024. H indicates which club is hosting the groups. Teams in bold advanced to the quarter-finals. The seeding was decided by as follows:
Group A.
Held in Sliema, Malta
Group B.
Held in Novaky, Slovakia
Group C.
Held in Sliema, Malta
Group D.
Held in Novaky, Slovakia
Knockout stage.
Draw.
The draw was on 11 November 2024. The seeded and unseeded clubs consist of the teams that finished first and second in the previous round respectively. The only restriction was that clubs from the same group in the previous round could not be drawn against each other. 
Bracket.
<section begin=Bracket /><section end=Bracket />
Quarterfinals.
Matches.
"ZV De Zaan won 36–15 on aggregate"
"EVK Zaibas won 24–22 on aggregate"
"GZC Donk won 38–25 on aggregate"
"Sliema ASC won 28–10 on aggregate"
Semifinals.
Matches.
"EVK Zaibas won 22–20 on aggregate"
"GZC Donk won 30–29 on aggregate"
Final.
Matches.
"GZC Donk won 23–22 on aggregate"