Methyl acetate hydrolase, known also as "methylacetate acetohydrolase" is a hydrolase enzyme that utilizes to carry out the hydrolysis of methyl acetate (CH3COOCH3), a weakly lipophillic and polar compound used often as a solvent. In the species "Gordonia sp." (strain TY-5), the enzyme is encoded by gene "acMB". The enzyme catalyzes the following reaction,
methyl acetate + = methanol + acetate + H+
The enzyme is involved in two major pathways, the butanoate metabolism pathway and the catabolic degradation pathway of propanol.