The 2000–01 FA Women's Cup was an association football knockout tournament for women's teams, held between 10 September 2000 and 7 May 2001. It was the 31st season of the FA Women's Cup and was won by Arsenal, who defeated Fulham in the final. The tournament consisted of two qualifying rounds and eight rounds of competition proper.
"All match results and dates from the Women's FA Cup Website."
Extra preliminary round.
All games were played on 10 and 17 September 2000.
Preliminary round.
All games were played on 24 September and 1 October 2000.
First round proper.
All games were scheduled for 29 October and 5, 10, 12 and 19 November 2000. 
Second round proper.
All games were originally scheduled for 3, 12, 19 and 26 November and 3 and 17 December 2000.
Third round proper.
All games were originally scheduled for 10 and 17 December 2000 and 7 and 14 January 2001.
Fourth round proper.
All games were originally scheduled for 7, 14 and 21 and 28 January 2001.
Fifth round proper.
All games were played on 28 January and 11 February 2001.
Quarter–finals.
All games were played on 18 February and 10 March 2001.
Semi–finals.
All games were played on 23 February 23.